|
مقاله
|
Abstract
|
|
|
Title:
|
Generation of Retinal Pigment Epithelium from Bone Marrow Derived Neurosphere in albino rat
|
Author(s):
|
Hamid Aboutaleb Kadkhodaeean, Taki Tiraihi1, Hamid Ahmadieh, Hosein ziaiiArdakani , NarsisDaftaryian.
|
Presentation Type:
|
Oral
|
Subject:
|
Posterior Segment
|
Others:
|
|
Presenting Author:
|
|
Name:
|
Hamid Aboutaleb Kadkhodaeean
|
Affiliation :(optional)
|
Shefa neuroscience research center ,Khatam-Al-Anbia Hospital, Tehran, Iran
|
E mail:
|
habootaleb92@gmail.com
|
Phone:
|
88454710
|
Mobile:
|
09377711768
|
|
|
Purpose:
|
INTRODUCTION:Retinal degenerations cause blindness. One potential therapy is cell replacement. Because the retina lacks regeneration capacity, much attention has been directed towards searching for cells that can differentiate into retinal neurons.
|
Methods:
|
METHODS:we could induce rat bone marrow-derived stem cells (BMSCs) into floating neurospheres. In addition to being able to differentiate into neuronal and RPE-like cells, neurospheres could be induced to differentiate into RPE-like cells. RPE-like cells were immunopositive for RPE 65.
|
Results:
|
RESULTS:immunocytochemistry of floating neurosphere were positive for nestin, MAP2, NF, O1. More than 90% of RPE-like cells were positive for RPE 65.
|
Conclusion:
|
CONCLUSIONS: these finding indicate that neurosphere derived from bone marrow steromal stem cells could differentiated to RPE-like cells in terms of morphology, phenotype and expression of specific markers. RPE-like cells induced from neurosphere may be useful for the treatment of aged related macular degeneration disease.
|
Attachment:
|
|
|