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مقاله
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Abstract
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Title:
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Calcein Fluorophotometry in Streptozocin-Induced Diabetic Rats
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Author(s):
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Reza Golshani, MD Gholam A.Peyman, MD, et al.
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Presentation Type:
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Poster
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Subject:
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Posterior Segment
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Others:
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Presenting Author:
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Name:
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Mohammadreza Golshani
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Affiliation :(optional)
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M.Reza Golshani,MD presented in ARVO
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E mail:
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rkgolshan@yahoo.com
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Phone:
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Mobile:
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0018183594369
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Purpose:
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Abstract
Purpose : Calcein was used in vitreous fluorophotometry (VF) to study the blood-retinal barrier(BRB) in rats with Streptozocin-induced diabetes.
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Methods:
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Materials and Methods : Calcein was injected intravenously (IV) in 18 diabetic rats and sodium fluorescein (NaF) in 12 diabetic rats. VF was performed at 1, 2, 4, 6, 9 and 21 hours following single injection in these diabetic rats; it was also performed at these times in 24 normal (control) rats. On another day, the same experiment was performed using NaF.
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Results:
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Results and Discussion : there was no significant difference between the two dyes in diabetic and non-diabetic rats at 1 hour. However, at 4 hours and at later measurement points, there was dye leakage into the vitreous, peaking at 9 hours; the leakage was significantly greater in the diabetic rats.Also, the leakage was significantly greater with Calcein than it was with NaF.
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Conclusion:
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Conclosion :The data demonstrates that Calcein circulates longer and at higher levels than NaF and thus is probably more useful for BRB integrity studies.
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Attachment:
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