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مقاله
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Abstract
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Title:
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Evaluation of transforming growth factor-beta1 gene expression in pterygium tissue of atopic patients
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Author(s):
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Mohammad Reza Shayegan MD,Hamid Gharaee MD, ,Mohammad Reza,Sina Kianoush ,A-Reza,Mojtaba Sankian
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Presentation Type:
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Poster
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Subject:
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Cornea and Anterior Segment
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Others:
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Presenting Author:
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Name:
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Hamid Gharaee
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Affiliation :(optional)
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Zakariya Research center,Mashhad Brunch,Slamic Azad University, Mashhad,IRAN,eye research center,Mashhad University of medical Sciences-
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E mail:
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gharaee_oph@yahoo.com
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Phone:
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8456918
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Mobile:
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09151117976
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Purpose:
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We compared the association between pterygium
and TGF-β1 gene expression between atopic patients and nonatopic
individuals.
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Methods:
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After obtaining informed
consents, questionnaires were used to obtain demographic and
clinical data from patients who underwent pterygium excision
surgery. Skin prick testing and serum IgE measurement were
done to confirm atopy in 30 consecutive patients (case group).
Besides, 30 consecutive patients without a history of atopy and
negative skin prick test were included as the control group. A
semiquantitative reverse transcriptase polymerase chain
reaction (RT-PCR) was done to determine TGF-β1 gene
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expression in all patients using glyceraldehyde 3-phosphate
dehydrogenase (GAPDH) gene as an internal control and the
results were compared between two groups.
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Results:
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Eosinophil count and serum IgE were significantly (P-values =
0.031 and 0.001, respectively) more in atopic patients
compared to the control group. Besides, TGF-?1 mRNA gene
expression was significantly (P-value = 0.0001) more in atopic
patients (2.50 ± 1.11) compared to non-atopic individuals (1.40
± 0.46).
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Conclusion:
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The excessive expression of TGF-β1
gene in pterygium tissue of patients with atopy suggests that
growth factors may play a role in the pathogenesis of
pterygium.
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Attachment:
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633sample .ppt
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